SARS-CoV-2 neutralising antibody testing in Europe: towards harmonisation of neutralising antibody titres for better use of convalescent plasma and comparability of trial data

Nguyen, Dung; Simmonds, Peter; Steenhuis, Maurice; Wouters, Elise; Desmecht, Daniel; Garigliany, Mutien; Romano, Marta; Barbezange, Cyril; Maes, Piet; Van Holm, Bram; Mendoza, Joaquin; Oyonarte, Salvador; Fomsgaard, Anders; Lassauniere, Ria; Zusinaite, Eva

VL / 26 - BP / - EP /
We compared the performance of SARS-CoV-2 neutralising antibody testing between 12 European laboratories involved in convalescent plasma trials. Raw titres differed almost too-fold differences between laboratories when blind-testing 15 plasma samples. Calibration of titres in relation to the reference reagent and standard curve obtained by testing a dilution series reduced the inter-laboratory variability ca to-fold. The harmonisation of neutralising antibody quantification is a vital step towards determining the protective and therapeutic levels of neutralising antibodies.

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Green published, Gold